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    A comparative study on the effectiveness of cis- and trans-form of cinnamic acid treatments for inhibiting invasive activit.pdf檔名:1426002656704.pdf
    篇名/題名:A comparative study on the effectiveness of cis- and trans-form of cinnamic acid treatments for inhibiting invasive activity of human lung adenocarcinoma cells
    摘要:Lung cancer is the major cause of tumor-related death, and approximately 70% of lung cancer patients die from metastasis. Evidence that phenolic compounds may have a potential inhibitory effect on cancer
    invasion and metastasis is increasingly being reported in the scientific literature. Cinnamic acid is a member of phenolics which having several isoforms in nature. The trans-cinnamic acid (t-CA) has been investigated
    extensively for its potential pharmacological effects whereas the study of cis-cinnamic acid (c-CA) is limited because pure c-CA was hard to obtain. We had developed a practicable method previously to
    transform and obtain pure c-CA, and the pure compound was used to evaluate the anti-invasive effect on human adenocarcinoma A549 cells. The A549 cells were treated with 0–200 lM of c-CA and t-CA in the presence of 200 nM phorbol-12-myristate-13-acetate (PMA) at 37 C for 24 h, and matrix metalloproteinase (MMP)-2, MMP-9, adhesive, migratory, and invasive activities of the cells were determined. The results showed that the treatment of c-CA and t-CA dose-dependently reduced the PMA-induced MMP-2 and -9 activities but without significant effect on the adhesive activity of cells. The PMA-induced motility was suppressed in a dose-dependent manner by a 24-h treatment with c-CA and t-CA. The invasive ability was significantly (p < 0.05) reduced to 68% and 65%, respectively, relative to PMA treatment
    alone after treatment of PMA-treated A549 cells with either 50 lM c-CA or 100 lM t-CA for 24 h. The Lung cancer is the major cause of tumor-related death, and approximately 70% of lung cancer patients die
    from metastasis. Evidence that phenolic compounds may have a potential inhibitory effect on cancer
    invasion and metastasis is increasingly being reported in the scientific literature. Cinnamic acid is a member
    of phenolics which having several isoforms in nature. The trans-cinnamic acid (t-CA) has been investigated
    extensively for its potential pharmacological effects whereas the study of cis-cinnamic acid (c-CA)
    is limited because pure c-CA was hard to obtain. We had developed a practicable method previously to
    transform and obtain pure c-CA, and the pure compound was used to evaluate the anti-invasive effect on
    human adenocarcinoma A549 cells. The A549 cells were treated with 0–200 lM of c-CA and t-CA in the
    presence of 200 nM phorbol-12-myristate-13-acetate (PMA) at 37 C for 24 h, and matrix metalloproteinase
    (MMP)-2, MMP-9, adhesive, migratory, and invasive activities of the cells were determined. The
    results showed that the treatment of c-CA and t-CA dose-dependently reduced the PMA-induced
    MMP-2 and -9 activities but without significant effect on the adhesive activity of cells. The PMA-induced
    motility was suppressed in a dose-dependent manner by a 24-h treatment with c-CA and t-CA. The invasive
    ability was significantly (p < 0.05) reduced to 68% and 65%, respectively, relative to PMA treatment
    alone after treatment of PMA-treated A549 cells with either 50 lM c-CA or 100 lM t-CA for 24 h. The
    results suggest that both of the c-CA and t-CA are inhibitors for invasion of A549 cells and the activity Lung cancer is the major cause of tumor-related death, and approximately 70% of lung cancer patients die
    from metastasis. Evidence that phenolic compounds may have a potential inhibitory effect on cancer
    invasion and metastasis is increasingly being reported in the scientific literature. Cinnamic acid is a member
    of phenolics which having several isoforms in nature. The trans-cinnamic acid (t-CA) has been investigated
    extensively for its potential pharmacological effects whereas the study of cis-cinnamic acid (c-CA)
    is limited because pure c-CA was hard to obtain. We had developed a practicable method previously to
    transform and obtain pure c-CA, and the pure compound was used to evaluate the anti-invasive effect on
    human adenocarcinoma A549 cells. The A549 cells were treated with 0–200 lM of c-CA and t-CA in the
    presence of 200 nM phorbol-12-myristate-13-acetate (PMA) at 37 C for 24 h, and matrix metalloproteinase
    (MMP)-2, MMP-9, adhesive, migratory, and invasive activities of the cells were determined. The
    results showed that the treatment of c-CA and t-CA dose-dependently reduced the PMA-induced
    MMP-2 and -9 activities but without significant effect on the adhesive activity of cells. The PMA-induced
    motility was suppressed in a dose-dependent manner by a 24-h treatment with c-CA and t-CA. The invasive
    ability was significantly (p < 0.05) reduced to 68% and 65%, respectively, relative to PMA treatment
    alone after treatment of PMA-treated A549 cells with either 50 lM c-CA or 100 lM t-CA for 24 h. The
    results suggest that both of the c-CA and t-CA are inhibitors for invasion of A549 cells and the activity Lung cancer is the major cause of tumor-related death, and approximately 70% of lung cancer patients die
    from metastasis. Evidence that phenolic compounds may have a potential inhibitory effect on cancer
    invasion and metastasis is increasingly being reported in the scientific literature. Cinnamic acid is a member
    of phenolics which having several isoforms in nature. The trans-cinnamic acid (t-CA) has been investigated
    extensively for its potential pharmacological effects whereas the study of cis-cinnamic acid (c-CA)
    is limited because pure c-CA was hard to obtain. We had developed a practicable method previously to
    transform and obtain pure c-CA, and the pure compound was used to evaluate the anti-invasive effect on
    human adenocarcinoma A549 cells. The A549 cells were treated with 0–200 lM of c-CA and t-CA in the
    presence of 200 nM phorbol-12-myristate-13-acetate (PMA) at 37 C for 24 h, and matrix metalloproteinase
    (MMP)-2, MMP-9, adhesive, migratory, and invasive activities of the cells were determined. The
    results showed that the treatment of c-CA and t-CA dose-dependently reduced the PMA-induced
    MMP-2 and -9 activities but without significant effect on the adhesive activity of cells. The PMA-induced
    motility was suppressed in a dose-dependent manner by a 24-h treatment with c-CA and t-CA. The invasive
    ability was significantly (p < 0.05) reduced to 68% and 65%, respectively, relative to PMA treatment
    alone after treatment of PMA-treated A549 cells with either 50 lM c-CA or 100 lM t-CA for 24 h. The
    results suggest that both of the c-CA and t-CA are inhibitors for invasion of A549 cells and the activity of c-CA seems to be higher than t-CA. of c-CA seems to be higher than t-CA. of c-CA seems to be higher than t-CA.
    results suggest that both of the c-CA and t-CA are inhibitors for invasion of A549 cells and the activity of c-CA seems to be higher than t-CA.
    類型:期刊論文
    西元出版年:2011
    著作語言:en_US
    關鍵詞:InvasionCinnamic acid
    作者:翁家瑞、Gow-Chin Yen、Yen-Ling Chen、Fang-Ming Sun、Yu-Ling Chiang、Shiau-Huei Lu、Chia-Jui Weng
    學校系所:生活應用科學碩士班